Table 3.
let-7a and let-7b expression and T-cell densities in colorectal cancer tissue
| let-7a expression | Univariate OR (95% CI) | Multivariate OR (95% CI) | let-7b expression | Univariate OR (95% CI) | Multivariate OR (95% CI) |
|---|---|---|---|---|---|
| Model of CD3+ cell density as a binary outcome (n = 514)a | |||||
| Q1 | 1 (reference) | 1 (reference) | Q1 | 1 (reference) | 1 (reference) |
| Q2 | 0.61 (0.37–1.01) | 0.62 (0.37–1.03) | Q2 | 1.82 (1.11–2.98) | 1.75 (1.06–2.88) |
| Q3 | 0.81 (0.49–1.36) | 0.82 (0.49–1.39) | Q3 | 1.89 (1.15–3.09) | 1.67 (1.01–2.78) |
| Q4 | 0.41 (0.25–0.68) | 0.40 (0.23–0.67) | Q4 | 1.40 (0.85–2.32) | 1.30 (0.78–2.18) |
| Ptrendb | 0.004 | 0.003 | Ptrendb | 0.18 | 0.40 |
| Model of CD8+ cell density as a binary outcome (n = 506)a | |||||
| Q1 | 1 (reference) | 1 (reference) | Q1 | 1 (reference) | 1 (reference) |
| Q2 | 1.08 (0.66–1.79) | 1.05 (0.63–1.76) | Q2 | 1.01 (0.61–1.66) | 1.03 (0.61–1.72) |
| Q3 | 0.81 (0.49–1.33) | 0.77 (0.46–1.29) | Q3 | 1.47 (0.90–2.41) | 1.47 (0.88–2.47) |
| Q4 | 0.55 (0.34–0.92) | 0.54 (0.32–0.91) | Q4 | 1.80 (1.09–2.99) | 1.59 (0.94–2.69) |
| Ptrendb | 0.010 | 0.008 | Ptrendb | 0.008 | 0.04 |
| Model of CD45RO+ cell density as a binary outcome (n = 518)a | |||||
| Q1 | 1 (reference) | 1 (reference) | Q1 | 1 (reference) | 1 (reference) |
| Q2 | 0.50 (0.29–0.87) | 0.50 (0.28–0.88) | Q2 | 1.22 (0.71–2.11) | 1.34 (0.76–2.36) |
| Q3 | 0.60 (0.35–1.03) | 0.52 (0.30–0.92) | Q3 | 1.18 (0.68–2.04) | 1.32 (0.75–2.31) |
| Q4 | 0.33 (0.18–0.60) | 0.31 (0.17–0.58) | Q4 | 0.48 (0.25–0.92) | 0.52 (0.27–1.00) |
| Ptrendb | 0.001 | 0.0004 | Ptrendb | 0.05 | 0.09 |
| Model of FOXP3+ cell density as a binary outcome (n = 491)a | |||||
| Q1 | 1 (reference) | 1 (reference) | Q1 | 1 (reference) | 1 (reference) |
| Q2 | 1.01 (0.61–1.68) | 1.07 (0.64–1.81) | Q2 | 1.12 (0.68–1.85) | 1.09 (0.65–1.83) |
| Q3 | 0.94 (0.57–1.57) | 0.89 (0.53–1.51) | Q3 | 1.26 (0.76–2.07) | 1.17 (0.70–1.96) |
| Q4 | 1.06 (0.64–1.76) | 1.00 (0.60–1.68) | Q4 | 0.76 (0.45–1.27) | 0.77 (0.46–1.32) |
| Ptrendb | 0.88 | 0.84 | Ptrendb | 0.40 | 0.43 |
The multivariate logistic regression model initially included age, sex, year of diagnosis, family history of colorectal cancer in parent or sibling, tumor location, microsatellite instability, CpG island methylator phenotype, KRAS, BRAF, and PIK3CA mutations, and LINE-1 methylation level. A backward stepwise elimination with a threshold of P < 0.05 was used to select variables in the final models.
Ptrend was calculated by the linear trend test across the ordinal categories (first to fourth quartile) of let-7a or let-7b expression as a continuous variable in the logistic regression model for the density of CD3+, CD8+, CD45RO+, or FOXP3+ cells as a binary outcome variable. We adjusted two-sided α level to 0.006 (= 0.05/8) by simple Bonferroni correction.
CI, confidence interval; Q1 to Q4, quartile 1 (lowest) to quartile 4 (highest).