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. 2016 Nov 18;10(11):e0005138. doi: 10.1371/journal.pntd.0005138

Fig 1. Diagnostic PCR assay of female-specific markers in males and females Schistosoma haematobium (S. h), Schistosoma bovis (S. b) and their hybrids.

Fig 1

The first three gels correspond to PCR amplifications on adult worms (biological duplicates for males and females) of the 5 female specific markers identified with our in silico approach. Note that upper-bands correspond to the GAPDH gene control (558 bp), whereas lower bands correspond to female-specific amplification (see Table 2 for amplicon sizes). Note also that amplification is not effective for amplification from the genome of S. bovis using the two markers WSh1 and Wsh2. The last two gels show sex-specific amplification for the three markers, efficient in both species on a batch of cercariae from molluscs mono-miracidially infected with S. haematobium.