Table 2. Summary information of the sex-specific markers identified.
| Name | Primer sequences (5’-3’) | Scaffold | Position | Amplicon size | Female specificity | ||
|---|---|---|---|---|---|---|---|
| S. h | S. b | S.h x S.b | |||||
| GAPDH | Forward | KL251991 (S. haematobium) SBOI.contig.33617.2504 (S. bovis) | 15,936–16,493 | 558 bp | No | No | No |
| CGACCATTGATGCAGCTAAA | |||||||
| Reverse | 1,000–1,557 | ||||||
| TTCCAAAATCCCCTTCATTG | |||||||
| WSh1 | Forward | KL253191 | 39–225 | 187 bp | Yes | NA | Yes |
| GCGTTCCGTTTAAAACATCG | |||||||
| Reverse | |||||||
| GTCCATGTGAGGGAATTTCG | |||||||
| WSh2 | Forward | KL252440 | 84–322 | 239 bp | Yes | NA | Yes |
| GAATCGATGACACTGGCGTA | |||||||
| Reverse | |||||||
| CCACTGTCCTTCGGAATTGT | |||||||
| WShSb1 | Forward | KL252782 | 3,138–3,334 | 197 bp | Yes | Yes | Yes |
| CCACTAGAGTCGTCGTCGTG | |||||||
| Reverse | |||||||
| GCTGCCGAATCCATAACAAA | |||||||
| WShSb2 | Forward | KL252782 | 8,258–8,487 | 230 bp | Yes | Yes | Yes |
| GTTGAAATTCGCTGCTGGAT | |||||||
| Reverse | |||||||
| AATGGTTTTGGACGGAATTG | |||||||
| WShSb3 | Forward | KL252440 | 18,445–18,637 | 193 bp | Yes | Yes | Yes |
| GGTGGTCAGGCATTGATTCT | |||||||
| Reverse | |||||||
| CATGTTTAGGCGCTTCAGGT | |||||||
NA: no amplification. S. h: Schistosoma haematobium. S. b: Schistosoma bovis