Table 1. Primers and PCR conditions used for DNA sequence amplifications in this study.

Gene	Primer name	Amplification primers (5'-3')	PCR Conditions	Amplicon size
16S rDNA	16s F	CGCCAGGGTTTTCCCAGTCACGACGCGTAGAGTTTGATCCTGGCTCAG	94°C 5 min; 94°C 1 min, 60°C 1 min, 72°C 1 min, 35 cycles; 72°C 10 min	1200 bp
	16s R	AGCGGATAACAATTTCACACAGGAGACGGGCGGTGTGTRCA
cpn60	H1594	CGCCAGGGTTTTCCCAGTCACGACGACGTCGCCGGTGACGGCACCACCAC	94°C 5 min; 94°C 30 s, 57°C 30 s, 72°C 45 s, 35 cycles; 72°C 10 min	555 bp
	H1595	AGCGGATAACAATTTCACACAGGACGACGGTCGCCGAAGCCCGGGGCCTT
gyrB	gyr-f	CGCCAGGGTTTTCCCAGTCACGACAAGCAGGGCAAGAGCGAGCTGTA	94°C 2.5 min; 94°C 30 s, 50°C 45 s, 72°C 1 min, 35 cycles; 72°C 7 min	600 bp
	gyr-r	AGCGGATAACAATTTCACACAGGACAAGGTGCTGAAGATCTGGTC
avrBs2	AvrBs2-F	CGCCAGGGTTTTCCCAGTCACGACGGACTAGTCCTGCCGGTGTTGATGCACGA	94°C 2 min; 94°C 1 min, 60°C 1 min, 72°C 1 min, 35 cycles; 72°C 7 min	780 bp
	AvrBs2-R	AGCGGATAACAATTTCACACAGGACCGCTCGAGCGGTGATCGGTCAACAGGCTTTC