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. 2016 Nov 14;11:6023–6033. doi: 10.2147/IJN.S115286

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© 2016 Ma et al. This work is published and licensed by Dove Medical Press Limited

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Fluorescence microscopy of new bone formation labeled by alizarin red and calcein after 4 and 8 weeks.

Notes: (A) Representative CLSM images. The red fluorescence is due to alizarin red labeling, and the yellow-green fluorescence is due to by calcein labeling. The scale bar is 100 μm. (B) Quantitative analysis of new bone formation. ^*Represents a significant difference compared to PEEK (P<0.01), and ^#represents a significant difference compared with n-HA/PEEK (^#P<0.05, ^##P<0.01).

Abbreviations: PEEK, polyetheretherketone; n-HA, nanohydroxyapatite; n-CS, nano-calcium silicate; CLSM, confocal laser scanning microscopy.