Table 1. Effects of MDMA, clorgyline, deprenyl, fluoxetine and the combinations on MTT reduction in P19 neurons.
| Compounds | 24 hours of incubation | 48 hours of incubation | ||||
|---|---|---|---|---|---|---|
| 37°C | 40°C | 42°C | 37°C | 40°C | 42°C | |
| Controls | 98±2.7 | 95±3.1 | 104±2.5 | 100±2.8 | 97±5.6 | 105±4.6 |
| 0.1% DMSO | 103±2.5 | 100±7.9 | 104±7.7 | 112±3.4 | 107±6.5 | 105±3.0 |
| 0.3 mM MDMA | 90±9.0 | 86±5.0 | 71±4.3*** | 93±6.2 | 86±6.4 | 73±8.3* |
| 1 mM MDMA | 66±4.6** | 61±4.4** | 33±6.1**** | 51±1.4**** | 67±3.7* | 42±13*** |
| 1 μM Clorg. | 95±8.0 | 90±7.0 | 104±3.3 | 108±3.7 | 101±7.1 | 105±8.1 |
| 1 μM Depr. | 110±3.6 | 103±6.2 | 112±5.6 | 112±7.9 | 113±3.8 | 113±6.2 |
| 1 μM Clorg.+ 1 μM Depr. | 103±7.5 | 104±8.9 | 106±4.3 | 103±5.9 | 101±12 | 112±4.3 |
| 10 nM Fluox. | 100±10 | 99±14 | 83±16 | 91±11 | 108±4.1 | 102±13 |
| 100 nM Fluox. | 92±13 | 94±9.9 | 73±16 | 87±9.7 | 94±10 | 85±11 |
| 1 μM Fluox. | 106±7.2 | 101±11 | 82±9.7 | 91±1.8 | 105±5.3 | 91±9.7 |
| 1 μM Clorg. + 1 μM Depr. + 1 μM Fluox. | 96±7.7 | 94±9.1 | 76±6.9 | 82±8.8 | 86±5.4 | 98±13 |
The compounds were added to the cells on day 7 in the serum-free medium followed by 24 h and 48 h incubation at 37°C, 40°C and 42°C, in a humified atmosphere with 5% CO2. Data (means ± SEM of four independent experiments) are expressed as percent of untreated controls or DMSO vehicle for fluoxetine. Statistical analysis was performed using one-way ANOVA with post hoc Dunnett’s multiple comparisons test against untreated controls or DMSO for fluoxetine (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).