Skip to main content
. 2016 Nov 18;11(11):e0166823. doi: 10.1371/journal.pone.0166823

Fig 6. Effect of Z-VAD-FMK on BNIP-3 expression and cytoplasmic AIF translocation to the nucleus after 12-h cadmium treatment.

Fig 6

(a) Representative images of BNIP-3 western blot. (b) Quantitative analysis of BNIP-3; grayscale of the control was set at 1. (c) Twelve-hour cadmium treatment with/without Z-VAD-FMK triggered AIF nuclear translocation. rPT cells were stained with anti-AIF antibodies and Alexa Fluor 488–labeled goat anti-rabbit IgG. AIF nuclear translocation was evaluated under fluorescence microscopy with DAPI staining. Scale bar: 50 μm. Results are from three independent experiments (mean ± SD, n = 3). **P < 0.01 as compared to control.