Strains |
|
|
P. chlororaphis subsp. aurantiaca PB-St2 |
wild type strain |
[13] |
E. coli XL1-Blue |
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIq ZΔM15 Tn10 (Tetr)] |
Stratagene |
Plasmids |
|
|
pBluescript SK(-) |
Cloning vector (phagemid excised from lambda ZAP). The f1 (–) orientation allows rescue of antisense strand ssDNA |
Stratagene |
pNH07 |
pBluescript SK(-) containing the inducible lac-promotor and aurI from P. aurantiaca PB-St2 at PsiI-PciI sites |
This study |
pNH08 |
pBluescript SK(-) containing the inducible lac-promotor and csaI from P. aurantiaca PB-St2 at PsiI-PciI sites |
This study |
pNH09 |
pBluescript SK(-) containing the inducible lac-promotor and phzI from P. aurantiaca PB-St2 at PsiI-PciI sites |
This study |
pNH10 |
pBluescript SK(-) containing the inducible lac-promotor and hdtS from P. aurantiaca PB-St2 at PsiI-PciI sites |
This study |
pNH11 |
pBluescript SK(-)ΔlacZ cut at PsiI-EcoRV sites |
This study |
pNH12 |
pBluescript SK(-) containing the inducible lac-promotor and aurI from P. chlororaphis subsp. aurantiaca StFRB508 at PsiI-PciI sites |
This study |
pNH13 |
pBluescript SK(-) containing the inducible lac-promotor and hdtS from P. fluorescens F113 at PsiI-PciI sites |
This study |