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. 2016 Nov 18;11(11):e0167002. doi: 10.1371/journal.pone.0167002

Table 1. Bacterial strains, plasmids, and primers used in this study.

Strain or plasmid Relevant characteristics Reference or source
Strains
P. chlororaphis subsp. aurantiaca PB-St2 wild type strain [13]
E. coli XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIq ZΔM15 Tn10 (Tetr)] Stratagene
Plasmids
pBluescript SK(-) Cloning vector (phagemid excised from lambda ZAP). The f1 (–) orientation allows rescue of antisense strand ssDNA Stratagene
pNH07 pBluescript SK(-) containing the inducible lac-promotor and aurI from P. aurantiaca PB-St2 at PsiI-PciI sites This study
pNH08 pBluescript SK(-) containing the inducible lac-promotor and csaI from P. aurantiaca PB-St2 at PsiI-PciI sites This study
pNH09 pBluescript SK(-) containing the inducible lac-promotor and phzI from P. aurantiaca PB-St2 at PsiI-PciI sites This study
pNH10 pBluescript SK(-) containing the inducible lac-promotor and hdtS from P. aurantiaca PB-St2 at PsiI-PciI sites This study
pNH11 pBluescript SK(-)ΔlacZ cut at PsiI-EcoRV sites This study
pNH12 pBluescript SK(-) containing the inducible lac-promotor and aurI from P. chlororaphis subsp. aurantiaca StFRB508 at PsiI-PciI sites This study
pNH13 pBluescript SK(-) containing the inducible lac-promotor and hdtS from P. fluorescens F113 at PsiI-PciI sites This study