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. 2016 Nov 18;11(11):e0167063. doi: 10.1371/journal.pone.0167063

Fig 8. Higher magnification images demonstrating interactions between microglia, Pax6+ cells, nerve fibers and blood vessels in the pineal gland.

Fig 8

Panels show confocal images of pineal gland (PG) immunolabeled for Pax6 (green), Iba1 (red) and β-tubulin III (Tuj1, blue), or IB4-stained blood vessels (v, blue). Microglia (yellow arrows) closely affiliate with and in some cases engulf Pax6+ precursor cells (yellow arrowheads) (A1-B4), Tuj1+ nerve fibers (white arrowheads) (C1-C4) and blood vessels (grey arrowheads) (D1-D4) within the PG. (A1-B3, C1-C3, D1-D3) 4x, 3.4x and 3.5x digital zooms from 60x images, respectively; scale bar: 5 μm. (A4, B4, C4, D4) 3D reconstruction of the main interactions.