Fig 8. Relative content of alpha-actinin in the membrane (MF) and cytoplasmic (CF) fractions.
(A) membrane fraction (MF). (B) cytoplasmic fraction (CF). The alpha-actinin content in the MF of the 3LS, 3LS+12h and 3LS+24h groups was higher than the MF of the 5LSS group by 54%, 63% and 57% (p<0.05), respectively, but remained unchanged in the CF. The alpha-actinin content in the MF of the 3LF group was the same as the MF of the 5LSS group but was reduced by 32% in the CF (p<0.05). The content in the MF of the 3LF+12h group was reduced by 32% (p<0.05), and in the CF, it was restored to the CF level of the 5LSS group. Then, the alpha-actinin content in the MF of the 3LF+24h group was increased by 35% compared with the MF of the 5LSS group (p<0.05), but it remained at the same level in the CF. The alpha-actinin content in the MF of the 5LSF group was the same as the MF of the 5LSS group, but in the CF, it was decreased by 26% (p<0.05). In the MF and CF of the 5LFF group, the alpha-actinin content was reduced by 27% and 39% compared with the MF and CF of the 5LSS group (p<0.05), respectively.