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. 2016 Sep 16;8(10):2308–2323. doi: 10.18632/aging.101038

Figure 1. Expression patterns of SIRT6 in young and senescent cells.

Figure 1

(A) Left, Western blot analysis of SIRT6 expression in young (Y, ≈PD 30), middle-aged (M, ≈PD 40) and senescent (O, ≈PD 55) 2BS cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against SIRT6, p16 as indicated. Tubulin served as a loading control. Right, the levels of SIRT6, p16 and TUBULIN in young (Y) and senescent (O) IMR90 cells were analysed by western blot analysis. (B) RT-PCR analysis of SIRT6 in young, middle-aged and senescent 2BS cells. Total mRNA was extracted and assessed by RT-PCR using specific primers. GAPDH was used as a loading control. (C) Protein levels of SIRT6 in indicated tissues of young (3 months) and old (18 months) BALB/c mice were determined with western blotting. Tubulin serves as loading control.