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. 2016 Sep 16;8(10):2308–2323. doi: 10.18632/aging.101038

Figure 8. SIRT6 expression correlates with p27 acetylation level and protein half-life during cellular senescence.

Figure 8

(A) Different amounts of SIRT6 were expressed in 293 cells. 48 h after transfection, SIRT6 and p27 were analysed by western blot. (B) Western blot analysis of SIRT6, p16 and p27 expression in young (Y, ≈PD 30), middle-aged (M, ≈PD 40) and senescent (O, ≈PD 55) 2BS and IMR90 cells. (C) The acetylation level of p27 was detected in young (Y, ≈PD 30) and senescent (O, ≈PD 55) 2BS cells. (D) The protein half-life of p27 during cellular senescence in 2BS was detected.