A- Endothelial-dependent relaxation induced by challenge with 0.5 μM carbachol. B- Endothelial-independent relaxation induced by challenge with 1 μM sodium nitroprusside. *P ≤ .05, ***P ≤ .001. Error bars represent standard error of the mean.
UnP, unprepared tissue; TraP, traditional preparation; ImP, improved preparation