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. Author manuscript; available in PMC: 2017 Dec 1.

Published in final edited form as: Hepatology. 2016 Oct 25;64(6):1978–1993. doi: 10.1002/hep.28811

Fig. 1 — Cell lysates were prepared from A. WT and IRAKM KO BMDMs and B. primary cultures of mouse Kupffer cells after they were untreated or treated with high dose LPS (1 µg/ml) and low dose LPS (100 pg/ml) for the indicated times and analyzed by Western blot analysis. C. Total mRNA from BMDMs of WT, IRAK1/2-DKO and IRAKM KO mice treated with low dose LPS (100 pg/ml) for the indicated times were subjected to RT-PCR analyses. D. Total mRNA from BMDMs of WT and IRAKM KO mice treated with low dose LPS (100 pg/ml) for the indicated times were subjected to RT-PCR analyses. E. BMDMs were treated with high dose LPS (1 µg/ml) or low dose LPS (100 pg/ml) for 24 hours. Cell-free supernatants were collected and cytokine concentrations were measured by ELISA assay. The experiments were repeated for five times with similar results. Data represent mean ± SEM; *, P<0.05.

Fig. 1 — Cell lysates were prepared from A. WT and IRAKM KO BMDMs and B. primary cultures of mouse Kupffer cells after they were untreated or treated with high dose LPS (1 µg/ml) and low dose LPS (100 pg/ml) for the indicated times and analyzed by Western blot analysis. C. Total mRNA from BMDMs of WT, IRAK1/2-DKO and IRAKM KO mice treated with low dose LPS (100 pg/ml) for the indicated times were subjected to RT-PCR analyses. D. Total mRNA from BMDMs of WT and IRAKM KO mice treated with low dose LPS (100 pg/ml) for the indicated times were subjected to RT-PCR analyses. E. BMDMs were treated with high dose LPS (1 µg/ml) or low dose LPS (100 pg/ml) for 24 hours. Cell-free supernatants were collected and cytokine concentrations were measured by ELISA assay. The experiments were repeated for five times with similar results. Data represent mean ± SEM; *, P<0.05.