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. Author manuscript; available in PMC: 2017 Dec 1.

Published in final edited form as: Hepatology. 2016 Oct 25;64(6):1978–1993. doi: 10.1002/hep.28811

Fig. 4 — A. Cell lysates from WT and IRAKM KO BMDMs untreated or treated with low dose LPS (100 pg/ml) for the indicated times were analyzed by Western blot analysis B. Cell lysates from WT and IRAK1/2 DKO BMDMs untreated or treated with low dose LPS (100 pg/ml) for the indicated times were analyzed by Western blot analysis. C. Immunostaining of F4/80 (green) and Mincle (red) was performed on frozen sections of liver from of WT and IRAKM KO mice (pair-fed and EtOH-fed). Confocal images are representative of five mice per group. D. Resident macrophages (F4/80⁺CD11b⁺) from livers of pair-fed and ethanol-fed mice were isolated by flow cytometry-based sorting. Total RNA was subjected to RT-PCR analysis. E. Tissue lysates from the whole liver of WT and IRAKM KO mice (pair-fed and EtOH-fed) were analyzed by Western blot analysis.

Fig. 4 — A. Cell lysates from WT and IRAKM KO BMDMs untreated or treated with low dose LPS (100 pg/ml) for the indicated times were analyzed by Western blot analysis B. Cell lysates from WT and IRAK1/2 DKO BMDMs untreated or treated with low dose LPS (100 pg/ml) for the indicated times were analyzed by Western blot analysis. C. Immunostaining of F4/80 (green) and Mincle (red) was performed on frozen sections of liver from of WT and IRAKM KO mice (pair-fed and EtOH-fed). Confocal images are representative of five mice per group. D. Resident macrophages (F4/80⁺CD11b⁺) from livers of pair-fed and ethanol-fed mice were isolated by flow cytometry-based sorting. Total RNA was subjected to RT-PCR analysis. E. Tissue lysates from the whole liver of WT and IRAKM KO mice (pair-fed and EtOH-fed) were analyzed by Western blot analysis.