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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Hepatology. 2016 Oct 25;64(6):1978–1993. doi: 10.1002/hep.28811

Fig. 6. SAP130-mediated mincle activation is required for low concentration LPS induced inflammation through inflammasome activation.

Fig. 6

Fig. 6

Fig. 6

A. Primary hepatocytes from WT mice were treated with EtOH (50 mM) for 24 hours. Cell-free supernatants were collected and SAP130 was measured by Western blot analysis. B. BMDMs from WT and Mincle KO mice were treated with PBS, LPS (100 pg/ml) for 24 hours, SAP130 (5 ug/ml) for 2 hours, TDB (100 µg/ml, 2 hours), LPS (100 pg/ml, 24 hours) + SAP130 (5 µg/ml, 2 hours) or LPS (100 pg/ml, 24 hours) + TDB (100 µg/ml, 2 hours). Total mRNA was subjected to RT-PCR analyses. C. BMDMs from WT and Mincle KO mice were treated with PBS, LPS (100 pg/ml) for 24 hours, LPS (100 pg/ml, 24 hours) + SAP130 (5 µg/ml, 6 hours) or LPS (100 pg/ml, 24 hours) + TDB (100 µg/ml, 6 hours). Cell lysates and supernatants were collected together and analyzed by Western blot. D. IL-1β was measured in Cell-free supernatants by ELISA. E. Tissue lysates from the whole liver of WT and IRAKM KO mice (pair-fed and EtOH-fed) were analyzed by Western blot. F. Tissue lysates from the whole liver of WT and Mincle KO mice (pair-fed and EtOH-fed) were analyzed by Western blot analysis.