Fig. 7.

Complement-mediated HCV binding to B cells worked on in vitro cultured HCV 1a and HCV 2a viruses. Three ml of the indicated virus (HCV1a, 1×10⁷ copies total; HCV2a, 1×10⁸ copies total; JFH1/1a, 3×10⁷ total) were treated either with 100 µl medium only as control or 100 µl serum at 25°C for 1 h, followed by mixing with 2 ml PBMCs (2.5×10⁷ cells per ml) in complete RPMI medium. The reaction was carried out at room temperature (25°C) for 2 h. The cells then were processed for HCV quantification as described in Methods section. ****: P < 0.0001 when compared to without serum treatment for each virus. Each value represents the mean ± SD of 6 determinations.