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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Hepatology. 2016 Aug 9;64(6):1881–1892. doi: 10.1002/hep.28705

Figure 1. Immunoprecipitation of HCV strains H77 (genotype 1a), J6 (2a), and S52 (3a) with and without HVR1 using HMAbs targeting five distinct antigenic regions of E2 or E1/E2.

Figure 1

Magnetic beads were coated with the indicated HMAbs and incubated with 106 IU HCV RNA from infectious cell culture supernatant containing (A) H77 or H77ΔHVR1, (B) J6 or J6ΔHVR1, or (C) S52 or S52ΔHVR1. The amount of associated HCV RNA was assessed by HCV RNA extraction directly from the beads and RT-qPCR in duplicates on the eluted fractions as described in Materials and Methods. Results are shown as mean of duplicates with SD (Cut-off: 800 IU). Star, value below cut-off.