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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Hepatology. 2016 Aug 9;64(6):1881–1892. doi: 10.1002/hep.28705

Figure 5. Unique envelope protein residues in neutralization resistant HVR1-deleted viruses conferred their phenotype.

Figure 5

The indicated viruses were incubated with a 5-fold dilution series of (A, C, E) AR5A, or (B, D, F) HC84.26 starting at 50 μg/ml or 5 μg/ml, respectively, along with eight replicates of virus only. 48h after inoculation of Huh7.5 cells with the virus/antibody mixes or virus only the number of FFU were visualized by HCV-specific immunostaining and the counts were normalized to the mean count of virus only. Three-parameter non-linear curve regression was used to fit the data points, top value set to 100 and bottom value set to 0 (Graphpad PRISM, v4.03). Error bars represent standard error of the mean. *the recombinant also had the dominant substitutions F291L, N532S, and D708V. #the recombinant also had the substitution I347L.