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Confocal images of confluent monolayers of WT and Cftr-KO cholangiocytes were acquired with the same imaging setting. Distibution of phalloidin (F-actin) staining reveals a defect in the proper formation of the cortical actin ring with accumulation of F-actin at the tri-cellular junctions in Cftr-KO cells compared with WT cells. E-cadherin was used to define the junctional and lateral cell areas.
