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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Hepatology. 2016 Oct 27;64(6):2118–2134. doi: 10.1002/hep.28817

Figure 6. Impaired epithelial barrier function in Cftr-KO monolayers.

Figure 6

A) Polarized monolayers of WT and Cftr-KO cholangiocytes were assayed for paracellular permeability to 10KDa FITC-dextrans. Concentration of dextrans diffused from the apical to the basolateral compartment was determined by linear regression (4-hour assay period) using known standard concentrations. Results represent the mean ± SD of 3 independent experiments in triplicate. B) WT and Cftr-KO cholangiocytes were seeded in transwell inserts. After reaching confluence TER was recorded daily for 9 days. Results represent the mean ± SD of 5 independent experiments.