. Author manuscript; available in PMC: 2017 Dec 1.

Published in final edited form as: Hepatology. 2016 Oct 28;64(6):1922–1933. doi: 10.1002/hep.28850

Table 1.

Binding kinetics of HC84.26 affinity mature clones against 1a H77C E2 and variants

	K_on (M⁻¹s⁻¹)			K_off (s⁻¹)			K_D (M)			K_D wt / K_D Matured
	1aE2	F442I E2	F442L E2	1aE2	F442I E2	F442L E2	1aE2	F442I E2	F442L E2	1aE2	F442I E2	F442L E2
HC84.26	1.3E+04	3.2E+03	5.6E+03	2.1E−04	6.3E−03	1.6E−03	1.6E−08	2.0E−06	2.8E−07	1.0	1.0	1.0
HC84.26.5	3.2E+04	1.3E+04	1.2E+04	2.5E−04	1.7E−03	1.1E−03	7.7E−09	1.3E−07	8.9E−08	2.1	15.7	3.2
HC84.26.5D	2.0E+04	1.1E+04	9.7E+03	1.3E−04	6.5E−04	4.4E−04	6.4E−09	5.8E−08	4.5E−08	2.5	34.0	6.3
HC84.26.5G	2.3E+04	1.3E+04	1.1E+04	8.5E−05	9.7E−04	6.7E−04	3.6E−09	7.7E−08	5.9E−08	4.4	26.0	4.8
HC84.26.5DG	2.7E+04	1.1E+04	1.0E+04	1.7E−04	8.7E−04	5.1E−04	6.3E−09	8.0E−08	4.9E−08	2.5	25.0	5.8