Figure 4. Co-exposure with HCV and HIV induces NFκB responsive genes in LX2 cells and Huh7.5.1 cells following mock treatment, HCV exposure, HIV exposure and HIV/HCV co-exposure for 72 hours.
Induction of NFκB responsive genes were as measured by (A) percentage of GFP-positive cells by flow cytometry; (B) geoMFI of GFP-positive cells by flow cytometry; and (C) phosphorylated RelA. The upper panels in part C shows representative western blots of the protein levels of phosphorylated RelA, total RelA, and β Actin. The lower panel of part C depicts densitometry quantification of the ratio of P-RelA to total RelA after exposure to HCV and/or HIV for 72 hours.
* P<0.05
HCV = hepatitis C virus; HIV = human immunodeficiency virus; GFP = green fluorescence protein; geoMFI = geometric fluorescence intensity; P-RelA = phosphorylated RelA