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. 2016 Nov 16;10(6):064106. doi: 10.1063/1.4966986

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© 2016 Author(s).

1932-1058/2016/10(6)/064106/9

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FIG. 1. — (a) Procedure for seeding cells. Cells are placed on an inverted Snapwell in 200 μl of media and allowed to adhere (b) the Snapwell is inverted into a 6-well plate for further growth. Note the cells are inverted at this point with the apical surface facing down. (c) The cell containing inset is separated from the support structure for insertion into the device. (d) Exploded view of the bioreactor. A PDMS microchannel defines the flow path. The Snapwell inset is secured in the lid of the bioreactor with a brace and sealed with a PDMS gasket. The inlet and outlet ports were tapped with 10–32 threaded luer fittings. (e) Assembled bioreactor (f) Cross-sectional view of the bioreactor (not to scale). The lid is designed such that the Snapwell insert sits flush with the flow path.