Table 3.
Possible near infrared FRET pairs with absorptions and emissions maxima, donor fluorescence quantum yield, acceptor molar extinction coefficient (providers’ data) and estimated Forster radius (using providers’ data in water or PBS).
Donor/acceptor pair | Donor absorbance (nm) | Donor emission (nm) | Acceptor absorbance (nm) | Acceptor emission (nm) | QY | L.mol−1.cm−1 | Förster Radius |
---|---|---|---|---|---|---|---|
AF633/AF660 | 632 | 647 | 663 | 690 | 0.3 | 132000 | 6.5 |
AF633/AF680 | 632 | 647 | 679 | 702 | 0.3 | 183000 | 7 |
AF650/AF700 | 650 | 668 | 702 | 723 | 0.33 | 192000 | 7 |
AF650/AF750 | 650 | 668 | 749 | 775 | 0.33 | 240000 | 6.5 |
AF660/AF750 | 663 | 690 | 749 | 775 | 0.37 | 240000 | 7 |
AF680/AF750 | 679 | 702 | 749 | 775 | 0.36 | 240000 | 7.5 |
AF680/IRdye800 | 679 | 702 | 774 | 789 | 0.36 | 240000 | 7 |
AF700/AF750 | 702 | 723 | 749 | 775 | 0.25 | 240000 | 7.5 |
AR700/IRdye800 | 702 | 723 | 774 | 789 | 0.25 | 240000 | 7 |
AF700/AF790 | 702 | 723 | 785 | 810 | 0.25 | 260000 | 7 |