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. 2016 Nov 15;7:13474. doi: 10.1038/ncomms13474

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Copyright © 2016, The Author(s)

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A series of replicate tubes containing the bistable system γtoγCy3.5/drainγ are prepared in the 0 state and repetitively switched ON—by addition of γ—and OFF—by addition of a degradable inhibitor (drainγ'). At each stage, all except one tube are actuated to prove the stability of the current state. The behaviour is monitored using both EvaGreen (total concentration of double-stranded DNA) and the red reporter Cy3.5 attached to the template, which shows a negative intensity shift when the template is in double-strand form²⁵. Each trace is the mean±2 s.d. over three replicates.