Table 2. Summary of catalase inactivation parameters in the presence or absence of various additives*.
Additives | Activity testkinact, min−1 |
Ultraviolet–visible spectroscopy |
|
---|---|---|---|
kobs(6), min−1 | kobs(11) × 103, min−1 | ||
None | 0.029±0.002 | 0.026±0.003 | 7.88±0.62 |
DMPO | 0.009±0.001 | 0.010±0.002 | 5.33±0.29 |
SOD | 0.018±0.001 | 0.020±0.002 | 6.73±0.39 |
Dimedone | †0.018±0.002 | †0.019±0.003 | 4.17±0.23 |
BCN | ND | 0.018±0.002 | 4.39±0.11 |
*The ultraviolet–visible spectroscopy experiments (n≥2±s.d.) and the activity tests (n=2±s.d.) were performed as described in ‘Methods' section.
†Effect due to the reactivity of dimedone with H2O2 and O2·−(see Supplementary Fig. 12).
ND, not determined.