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. 2016 Nov 18;23:81. doi: 10.1186/s12929-016-0296-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — SAHA causes cell cycle arrest of GSCs. a GSCs from U87MG cells were treated with SAHA (1 μM and 2.5 μM) for 24 h. The spheres were harvested for PI staining and analyzed by using flow cytometry. b Data regarding the population of sub-G1, G1, S, G2/M and > 4 N upon SAHA treatment was presented as the mean ± S.D. of experiments performed in triplicate; bar, S.D.; *p < 0.05, **p < 0.01. c The expression levels of cyclin B1, CDK1, PCNA, and p21 were measured by Western blotting. Tubulin was used as a loading control