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. 2016 Nov 18;9:590. doi: 10.1186/s13071-016-1870-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Validation of ICount egg counting approach. a Calibration curve of Manual vs Automatic counting of microscopic pictures of eggs (n = 380). Y_micro = 0.87x_micro + 3.02; R ² = 0.965, P < 0.0001. b Calibration curve of Manual vs Automatic counting of camera “macro” images of eggs (n = 48). Y_macro = 0.59x_macro + 69.74, R ² = 0.965, P < 0.0001