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. 2016 Nov 18;23:82. doi: 10.1186/s12929-016-0297-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Autophagy-deficiency decreases the advantage of CD271+ OS cells in terms of tumorigenesis in vitro. a–d The colony-forming and sphere-forming abilities of the indicated SaoS2 (a, b) and MNNG/HOS (c, d) cells were detected. The mean number of colonies (a, c) and spheres (b, d) per well and the mean volume of spheres in the indicated groups are shown in graphs. The data from three replicates are shown as the mean ± S.D. e The protein expression of Nanog, Oct3/4, Stat3 and p-Stat3 in the indicated OS cells were examined by western blotting