Fig. 5.

Biochemical analysis of SDS-soluble fraction of the gastrocnemius. a Coomassie stain of homogenate (25 μg protein) for Fo. 1579, 1576, and 1573. Average age for indicated group is reported months (m). All Tg mice had similar phenotypes at the age analyzed. Tg mice with a phenotype from line 1579 have a striking change in protein banding pattern compared to NT mice including a nearly complete loss of high MW (~170 kDa) band which may represent Myosin Heavy Chain. In contrast, banding pattern for phenotypic Tg mice from lines 1576 and 1573 were similar to NT mice on the Coomassie-stained gel. b Matrin 3 immunoblotting (15 μg protein) from NT and phenotypic Tg mice shown in Coomassie blot (a) shows the consistent presence of lower molecular weight bands in affected 1579 mice. c Matrin 3 immunoblotting (20 μg protein) from non-phenotypic, Tg mice (~2 m of age) from Fo. 1576 and 1573 show lower molecular weight bands similar that that observed in phenotypic mice from line 1579. Bottom panel is GAPDH as a loading control. * indicates homogenate from phenotypic Fo. 1576 age ~5 m was used as a reference sample. Arrow head indicates major Matrin 3 product ~120 kDa that often runs as a doublet