Table 3.
Acetoacetyl-CoA reductase (AAR) specific activity
Strain | Acetoacetyl-CoA reductase | Cofactor | Vmax (µmol/min mg total protein) |
---|---|---|---|
TMB4443 | C. necator | NADH | ND |
NADPH | 9.9 ± 3.9 | ||
TMB4445 | A. vinosum | NADH | 184.7 ± 8.6 |
NADPH | 38.6 ± 2.2 |
200 µM of acetoacetyl-CoA and crude extracts of S. cerevisiae strains from overnight cultures cultivated in YNB-glucose were used. Averages and standard deviations were obtained from duplicate experiments
ND not detected