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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Immunol. 2016 Oct 31;197(11):4493–4503. doi: 10.4049/jimmunol.1600576

Figure 6.

Figure 6

Tumor-associated inflammation in SCLC. (A) Rbp53 mice develop SCLC tumors within 1 year of AdCre exposure. H&E section, scale bar = 1 mm. (B) Flow cytometric analysis of lungs from tumor-bearing Rbp53 mice (n = 4) and non-Cre exposed control mice (n = 3), approximately 10 months after tumor induction. IHC staining reveals that CD45+ immune cells are generally located in the SCLC tumor periphery (arrowheads, C), with some clustering of cells into organized lymphoid structures (arrows, D). Some large CD45+ cells, likely macrophages, were observed in alveolar spaces (arrowheads, D) proximal to the tumor. Little to no leukocyte tumor infiltration was observed (E). Scale bars = 1 mm (C) and 100 μm (D, E). (F) The ratio of CD3+ T cells to sum myeloid population in SCLC mice was significantly increased compared to three ADCA models, as assessed by one-way ANOVA with Tukey's post-test. (G) The percentage of CD45+ live cells present in two resected specimens of human SCLC was greatly reduced compared to five human ADCA specimens. (H) Leukocyte population summary of the flow cytometric analyses, shown as percent of live cells, for a representative normal mouse lung, 10-week Egfr, Kras, and Kp53 ADCA and mouse SCLC are displayed. Abbreviations: NL, non-adjacent normal lung; Tu, tumor. Asterisks indicate P < 0.05.