Table 3.
The effect of native lactoferrin (N) on LPS-induced secretion of IL-6 and TNF-α by J774A.1 cells
| Sample | IL-6 | TNF-α | ||
|---|---|---|---|---|
| Concentration (pg/ml) | Maximal response (%) | Concentration (pg/ml) | Maximal response (%) | |
| LPS | 22,280 ± 5175 | 100 | 20,536 ± 2354 | 100 |
| N + LPS | 700 ± 100 | 2.8 ± 0.4 | 4873 ± 1199 | 23.7 ± 5.8 |
| LPS + N | 785 ± 35 | 3.1 ± 0.1 | 2260 ± 381 | 11.0 ± 1.8 |
| LPS (3 h) + N | 6685 ± 1180 | 26.6 ± 4.7 | 11,350 ± 537 | 55.2 ± 2.6 |
| N- > LPS* | 21,086 ± 1766 | 83.81 ± 7.0 | 10,640 ± 714 | 51.8 ± 3.5 |
The results are expressed as a percentage of maximal response (mean ± SEM from triplicates assayed in one of two independent experiments)
N + LPS denotes that LPS was added 30 min after N was added to the cells
LPS + N denotes that LPS was added 30 min prior to N addition
LPS(3 h) + N denotes that incubation with LPS was extended to a period of 3 h
N- > LPS* denotes that the medium was changed after the nLf incubation and LPS was added to nLf-free medium