Figure 4.

Endogenous n-3 PUFA protects against CCl₄-induced hepatocyte apoptosis in fat-1 mice via regulating MAPK signaling pathway. (a) Representative images of TUNEL stained liver sections (magnification: 200x): green fluorescence indicates the positive cells, and cellular nucleus is labeled by staining DAPI with blue fluorescence. (b) Western blot analysis of apoptosis-related proteins, including cytochrome C, caspase-3, caspase-9, Bcl-2, and Bax. (c) Western blot analysis of total and phosphorylated protein expression of JNK, p38, and ERK.