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. Author manuscript; available in PMC: 2017 Aug 4.
Published in final edited form as: Cell Stem Cell. 2016 May 6;19(2):258–265. doi: 10.1016/j.stem.2016.04.014

Figure 2.

Figure 2

RNA map of Cerebral Organoids development. (A) Heat map of transcriptome analysis from human embryonic stem cells (Group 1) and cerebral organoids after 1 month (Group 2) and 2 months (Group 3) in culture show 3226 and 3357 significantly differentially expressed genes with fold change >2, p-value <0.05. See also Figure S2A. See Table 1. (B) Gene ontology analysis show top 10 more enriched terms for upregulated (top) and downregulated (bottom) genes during cerebral organoid differentiation. (C) Grouped functional pathway analysis of differentially expressed genes during organoid formation. (D) Heat map of differentially expressed genes between organoids 1 month (Group 2) and 2 months (Group 3) in culture. Group 1 represents human embryonic stem cells. (E) Gene ontology analysis of differentially expressed genes in organoids 1 and 2 months old suggest formation of retinal tissue. (F–G) Spearman’s correlation heat map of cerebral organoid transcriptomes compared with regions of the fetal brain and age post conception weeks (pcw). See Table 2 for heat map brain region legend. Also see Figure S2B and S2C.