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. Author manuscript; available in PMC: 2017 Oct 17.
Published in final edited form as: Chem Res Toxicol. 2016 Sep 21;29(10):1741–1754. doi: 10.1021/acs.chemrestox.6b00244

Table 6.

Steady-State Kinetic Parameters for dNTP Incorporation Opposite 8-oxoG and O6-MeG by human wild-type κ (1–526) and variants

template pol κ(1–526) dNTP Km (µM) kcat (s−1) kcat/Km (s−1 µM−1) finsa relative
efficiencyb
8-oxoG wild-type A 6.9 ± 0.7 0.37 ± 0.01 5.4 × 10−2 4.2 1
C 42 ± 7 0.56 ± 0.03 1.3 × 10−2 1
E29K A 6.6 ± 0.8 0.37 ± 0.01 5.6 × 10−2 5.1 1.0
C 41 ± 5 0.47 ± 0.02 1.1 × 10−2 1
T44M A 12 ± 3 0.45 ± 0.03 3.8 × 10−2 3.9 0.70
C 96 ± 9 0.94 ± 0.03 9.8 × 10−3 1
F192C A 5.8 ± 0.6 0.31 ± 0.01 5.3 × 10−2 4.4 1.0
C 48 ± 6 0.57 ± 0.02 1.2 × 10−2 1
R246X A 9.1 ± 0.8 0.10 ± 0.01 1.1 × 10−2 2.6 0.20
C 65 ± 12 0.27 ± 0.01 4.2 × 10−3 1
E292K A 4.1 ± 0.8 0.24 ± 0.01 5.9 × 10−2 6.4 1.1
C 51 ± 8 0.47 ± 0.02 9.2 × 10−3 1
R298H A 22 ± 3 0.26 ± 0.01 1.2 × 10−2 8.0 0.22
C 400 ± 60 0.59 ± 0.03 1.5 × 10−3 1
A471V A 12 ± 3 0.25 ± 0.02 2.1 × 10−2 4.2 0.39
C 110 ± 20 0.55 ± 0.03 5.0 × 10−3 1
T473A A 50 ± 9 0.22 ± 0.01 4.4 × 10−3 1.1 0.081
C 150 ± 20 0.58 ± 0.02 3.9 × 10−3 1
R512W A 120 ± 10 0.17 ± 0.01 1.4 × 10−3 0.93 0.026
C 270 ± 60 0.41 ± 0.03 1.5 × 10−3 1
O6-MeG wild-type T 230 ± 40 0.48 ± 0.02 2.1 × 10−3 0.40
C 110 ± 30 0.55 ± 0.05 5.0 × 10−3 1 1
E29K T 350 ± 40 0.46 ± 0.02 1.3 × 10−3 0.30
C 170 ± 30 0.76 ± 0.04 4.5 × 10−3 1 0.90
T44M T 180 ± 40 0.047 ± 0.003 2.6 × 10−4 0.54
C 280 ± 30 0.13 ± 0.01 4.6 × 10−4 1 0.092
F192C T 210 ± 30 0.33 ± 0.01 1.6 × 10−3 0.39
C 140 ± 20 0.56 ± 0.02 4.0 × 10−3 1 0.80
R246X T 210 ± 30 0.16 ± 0.01 7.6 × 10−4 0.43
C 150 ± 30 0.26 ± 0.01 1.7 × 10−3 1 0.34
E292K T 150 ± 40 0.30 ± 0.02 2.0 × 10−3 0.37
C 130 ± 20 0.62 ± 0.02 4.8 × 10−3 1 0.96
R298H T 560 ± 110 0.13 ± 0.01 2.3 × 10−4 0.51
C 690 ± 60 0.31 ± 0.01 4.5 × 10−4 1 0.090
A471V T 270 ± 70 0.35 ± 0.03 1.3 × 10−3 0.49
C 230 ± 40 0.61 ± 0.03 2.7 × 10−3 1 0.54
T473A T 280 ± 50 0.18 ± 0.01 6.4 × 10−4 0.47
C 190 ± 60 0.26 ± 0.02 1.4 × 10−3 1 0.28
R512W T 250 ± 60 0.033 ± 0.002 1.3 × 10−4 0.64
C 460 ± 80 0.096 ± 0.006 2.1 × 10−4 1 0.042
a

Misinsertion frequency, calculated by dividing kcat/Kmfor each dNTP incorporation by the kcat/Km for dCTP incorporation opposite template base.

b

Relative efficiency, calculated by dividing kcat/Km of each pol κ(1–526) for dNTP incorporation opposite template base by the highest kcat/Km of wild-type pol κ(1–526) for dNTP incorporation opposite template base.