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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cell Microbiol. 2016 Jul 11;18(12):1782–1799. doi: 10.1111/cmi.12617

Figure 2. In vitro phenotypes of the ΔcheY3.

Figure 2

A. ΔcheY3 colonies exhibit a significantly reduced swarming ability on semi-solid agar plates when compared to the parental WT or complemented cells (P = 0.0001). Values are mean ± SD from at least 11 individual colonies per strain. Statistical analysis was performed by using ANOVA followed by Tukey’s Multiple Comparisons test. A P<0.05 between strains is considered significant. B. The ΔcheY3 is deficient in chemotaxis as determined by the capillary tube chemotaxis assay using N-Acetylglucosamine as an attractant. A capillary tube filled with buffer without any attractant was used as a control. An increase in the number of spirochetes equal to or greater than twice that of the buffer control was considered “chemotactic”. The numbers on top of each vertical bar is the fold-increase over the buffer control. Results shown are mean ± SEM from three independent studies with three replicates per strain per assay.