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. 2016 Nov 21;7:1712. doi: 10.3389/fpls.2016.01712

FIGURE 4.

FIGURE 4

AtHMAD1 negatively regulates the basal defense system. Pathogen growth from the inoculated leaves with virulent Pst DC3000 at 5 × 105 colony forming units (CFU) mL-1after 2 days post inoculation (DPI; A). Development of symptoms in wild type (WT) and transgenic lines following infiltration with virulent Pst DC3000 (B). Transcript accumulation of the PR gene (SA-dependent pathway) in the indicated Arabidopsis genotypes in response to pressure infiltration of Pst DC3000 using qPCR (C). The expression values were normalized using the constitutively expressed actin. All data points represent the mean of four replicates. Error bars represent ± SE (n = 4) with asterisks indicate highly significant differences (99% confidence level) from WT plants. The experiment was repeated three times with similar results (Student’s t-test, P < 0.0000265).