Gene Knockout: rewriting of host DNA |
A. thaliana |
Stable integration |
RTEL1(Regulator Of Telomere Elongation Helicase 1) |
RTEL1 functions in DNA replication, DNA repair, and recombination |
Schiml et al., 2014 |
|
A. thaliana |
Stable integration |
AP1 (floral homeotic gene APETALA1), BRI1 (Brassinosteroid-insensitive2), CHLI1(Magnesium-chelatase subunit ChlI-1) |
BRI1 encodes a cell surface receptor for brassinosteroids.AP1 Encodes a putative TF that acts locally to specify the identity of the floral meristem. CHLI1 plays role in chlorophyll biosynthesis. |
Feng et al., 2014 |
|
A. thaliana |
Stable integration |
ADH1 (Alcohol dehydrogenase class-P) |
ADH1 is required for survival and acclimation in hypoxic conditions, especially in roots |
Fauser et al., 2014 |
|
A. thaliana N. benthamiana |
Protoplasts, Agrobacterium T-DNA(Transient) |
At Phytoene desaturase gene (PDS3), NbPDS3
|
PDS3 is needed for primary carbon and pigment metabolism. Its activity acts as a rheostat of retrograde signaling during early chloroplast biosynthesis. |
Li et al., 2013 |
|
O. sativa, S. bicolor, |
Protoplasts, Agrobacterium T-DNA (Transient) |
OsSWEET14, |
OsSWEET14 id needed for disease resistance. |
Jiang et al., 2013 |
|
O. sativa T. aestivum |
Protoplasts |
OsPDS,OsBADH2 (betaine aldehyde dehydrogenase-2), OsMPK2 (ortholog of tobacco SIPK), TaMLO (Mildew-resistance locus) |
BADH2
plays important role in abiotic stress tolerance.
OsMPK2 is activated by elicitors It negatively regulates the expression of defense-related genes. TaMLO in plant confers heritable broad-spectrum resistance to powdery mildew |
Shan et al., 2013c |
|
A. thaliana, O. sativa |
Stable integration |
AtJAZ1 (Jasmonate ZIM-domain), (Gibberellic Acid insensitive),OsROC5 (RICE OUTMOST CELL-SPECIFIC GENE 5), OsSPP, OsYSA
|
AtJAZ1 is transcription repressor of jaJA)-responsive genes and major components of theJA receptor complex. AtGAI
is important in attenuation of GA responses. |
Feng et al., 2013 |
|
O. sativa |
Stable integration |
OsWEET11/13/1a/1b |
OsSWEET14 id needed for disease resistance. |
Zhou et al., 2014 |
Large deletions: |
A. thaliana |
Protoplasts |
PDS3 |
PDS3 is needed for primary carbon and pigment metabolism. |
Li et al., 2013 |
Gene replacement: |
O. sativa |
Protoplasts |
Phytoene desaturase gene |
PDS3 is needed for primary carbon and pigment metabolism. |
Shan et al., 2013a |
|
N. benthamiana |
Protoplasts |
Phytoene desaturase gene |
PDS3 is needed for primary carbon and pigment metabolism. |
Li et al., 2013 |
|
A. thaliana |
Stable integration |
ADH1 (Alcohol dehdrogenase1) |
ADH1 is required for survival and acclimation in hypoxic conditions, especially in roots |
Schiml et al., 2014 |
Controlling gene expression |
N. benthamiana |
Agrobacterium T-DNA (Transient) |
PDS3 |
PDS3 is needed for primary carbon and pigment metabolism. Its activity acts as a rheostat of retrograde signaling during early chloroplast biosynthesis. |
Piatek et al., 2014 |