Skip to main content
. 2016 Nov 21;7:1740. doi: 10.3389/fpls.2016.01740

Table 1.

Successful application of CRISPR-Cas9 in different plant species.

DNA modification type Plant Delivery mode Target(s) Gene function(s) Reference
Gene Knockout: rewriting of host DNA A. thaliana Stable integration RTEL1(Regulator Of Telomere Elongation Helicase 1) RTEL1 functions in DNA replication, DNA repair, and recombination Schiml et al., 2014
A. thaliana Stable integration AP1 (floral homeotic gene APETALA1), BRI1 (Brassinosteroid-insensitive2), CHLI1(Magnesium-chelatase subunit ChlI-1) BRI1 encodes a cell surface receptor for brassinosteroids.AP1 Encodes a putative TF that acts locally to specify the identity of the floral meristem. CHLI1 plays role in chlorophyll biosynthesis. Feng et al., 2014
A. thaliana Stable integration ADH1 (Alcohol dehydrogenase class-P) ADH1 is required for survival and acclimation in hypoxic conditions, especially in roots Fauser et al., 2014
A. thaliana N. benthamiana Protoplasts, Agrobacterium T-DNA(Transient) At Phytoene desaturase gene (PDS3), NbPDS3 PDS3 is needed for primary carbon and pigment metabolism. Its activity acts as a rheostat of retrograde signaling during early chloroplast biosynthesis. Li et al., 2013
O. sativa, S. bicolor, Protoplasts, Agrobacterium T-DNA (Transient) OsSWEET14, OsSWEET14 id needed for disease resistance. Jiang et al., 2013
O. sativa T. aestivum Protoplasts OsPDS,OsBADH2 (betaine aldehyde dehydrogenase-2), OsMPK2 (ortholog of tobacco SIPK), TaMLO (Mildew-resistance locus) BADH2 plays important role in abiotic stress tolerance. OsMPK2 is activated by elicitors It negatively regulates the expression of defense-related genes. TaMLO in plant confers heritable broad-spectrum resistance to powdery mildew Shan et al., 2013c
A. thaliana, O. sativa Stable integration AtJAZ1 (Jasmonate ZIM-domain), (Gibberellic Acid insensitive),OsROC5 (RICE OUTMOST CELL-SPECIFIC GENE 5), OsSPP, OsYSA AtJAZ1 is transcription repressor of jaJA)-responsive genes and major components of theJA receptor complex. AtGAI is important in attenuation of GA responses. Feng et al., 2013
O. sativa Stable integration OsWEET11/13/1a/1b OsSWEET14 id needed for disease resistance. Zhou et al., 2014
Large deletions: A. thaliana Protoplasts PDS3 PDS3 is needed for primary carbon and pigment metabolism. Li et al., 2013
Gene replacement: O. sativa Protoplasts Phytoene desaturase gene PDS3 is needed for primary carbon and pigment metabolism. Shan et al., 2013a
N. benthamiana Protoplasts Phytoene desaturase gene PDS3 is needed for primary carbon and pigment metabolism. Li et al., 2013
A. thaliana Stable integration ADH1 (Alcohol dehdrogenase1) ADH1 is required for survival and acclimation in hypoxic conditions, especially in roots Schiml et al., 2014
Controlling gene expression N. benthamiana Agrobacterium T-DNA (Transient) PDS3 PDS3 is needed for primary carbon and pigment metabolism. Its activity acts as a rheostat of retrograde signaling during early chloroplast biosynthesis. Piatek et al., 2014