Table 5.
Different plasmids with their genes, vectors, and promoters used in CRISPR-Cas9 technique.
| Plasmids | Gene/Insert | Promoter | Vector type | Vector back bone | Purpose | Bacterial resistance | Reference |
|---|---|---|---|---|---|---|---|
| pK7WGF2::hCas9 | hCas9 (Syn) | 35S | Plant expression, CRISPR | pK7WGF2, | Expresses the human codon by using Cas9 with N-terminal GFP tag from the 35S promoter in the plant tissue | Spectinomycin | Nekrasov et al., 2013 |
| PHSE401 | zCas9, gRNA scaffold | 35S, AtU6-26p | Plant Expression; plant binary vector | pCambia, | CRISPR/Cas based plant genome editing and gene regulation | Kanamycin | Xing et al., 2014 |
| pHEE401E | zCas9, gRNA scaffold | U6-26p Arabidopsis U6 gene promoter, EC1.2 enhancer fused to EC1.1 promoter | Plant Expression; plant binary vector, CRISPR | pCambia | Contain gRNA scaffold for insertion of target sequence, Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS | Kanamycin | Wang et al., 2015 |
| pHSN501 | zCas9D10A, gRNA scaffold (Syn) | AtU6-26p, 2 × 35Sp | CRISPR; Plant expression | pGreen-like binary vector | CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A | Kanamycin and Spectinomycin | Xing et al., 2014 |
| pBUN501 | gRNA scaffold (Syn), zCas9D10A (Syn) | AtU6-26p, Ubi1p, | Plant expression, CRISPR | pGreen-like binary vector | CRISPR/Cas based plant genome editing and gene regulation; | Kanamycin and Spectinomycin | Xing et al., 2014 |
| pHSN6A01 | gRNA scaffold (Syn),dCas9-VP64 (Syn) | AtU6-26p, 2 × 35Sp, | Plant expression, CRISPR | pGreen-like binary vector | expresses dCas9-VP64, gRNA scaffold for insertion of target sequence | Kanamycin and Spectinomycin | Xing et al., 2014 |
| pBUN6A11 | gRNA scaffold (Syn), dCas9-VP64 (Syn), | OsU3p, Ubi1p, | Plant expression, CRISPR | pGreen-like binary vector | expresses dCas9-VP64, gRNA scaffold for insertion of target sequence | Kanamycin and Spectinomycin | Xing et al., 2014 |
| pEGB 35s:dCas:BRD:tNos (GB1172) | dCas9:BRD | 35S | Plant expression, CRISPR | pDGB3alpha2 | Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor | Kanamycin | Vazquez-Vilar et al., 2016 |
| pEGB 35S:dCas9:Tnos (GB1191) | dCas9 | 35S | Plant Expression, CRISPR, Synthetic Biology | pDGB3alpha2 | Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter | Kanamycin | Sarrion-Perdigones et al., 2013 |
| pJIT163-2NLSCas9 | dCas9 | 2 × 35S | Plant Expression | pJIT163 | Expression of rice codon-optimized Cas9 in plant cells | Ampicillin | Shan et al., 2013b |
| HBT-pcoCas9 | Pro Cas9 (syn) | Hybrid constitutive promoter 35SPPDK | CRISPR; Plant expression | HBT-FLAG | Transient expression of pcoCas9 gene in plant cells | Ampicillin | Li et al., 2013 |