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. 2016 Nov 21;7:1740. doi: 10.3389/fpls.2016.01740

Table 6.

Specific commercial products and services available to the researchers to implement CRISPR technology.

Commercial sources Products and services
GeneCopoeia Genome-wide sgRNA clones HDR donor cloning vectors and custom HDR donor construction. Cas9 stable cell lines. Insertion/deletion detection system
Sigma–Aldrich CRISPR Selection Too Paired nickases Codon-optimized Cas9 Transfection-grade CRISPR plasmid with a guide RNA
Bio Labs England Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) EnGe Cas9 Nuclease EnGen Mutation Detection Kit
Integrated DNA technologies (IDT) Human HPRT PCR Primer Mix, Mouse HPRT PCR Primer Mix, Nuclease Free Duplex Buffer S.p. Cas9 Expression Plasmid S.p. Cas9 Nuclease 3NLS (100, 500 μg) CRISPR Negative Control crRNA, CRISPR Positive Control crRNA
DNA 2.0 Nickase Ninja All-in-One construct expressing specific dual gRNAs Electra Cloning Kit
Cyagen ROSA26 large fragment knockin
ORiGene CRISPR/Cas starter kit (HA tagging human HSP60 at C-terminus). pCas-Guide-Nickase (D10A), pT7-Cas9-Nickase (D10A) pCas-Guide Cloning Kit, pCas-Guide-scramble (also available as negative control)
System Biosciences Multiplex gRNA Cloning Kit Create CRISPR/Cas9 constructs with multiple gRNAs simultaneously for better genome editing
Eurofins Genomics Cloning Oligos Indel Detection by Amplicon Analysis Custom Sequencing (Check the sequence of your CRISPR plasmid or genomic target sequence)

This table is provided only to facilitate researchers and R&D. It does not involve any commercial affiliation, marketing or financial purpose.