Table 6.
Commercial sources | Products and services | |||
---|---|---|---|---|
GeneCopoeia | Genome-wide sgRNA clones | HDR donor cloning vectors and custom HDR donor construction. | Cas9 stable cell lines. | Insertion/deletion detection system |
Sigma–Aldrich | CRISPR Selection Too | Paired nickases | Codon-optimized Cas9 | Transfection-grade CRISPR plasmid with a guide RNA |
Bio Labs England | Q5 Hot Start High-Fidelity 2X Master Mix, NEBuilder HiFi DNA Assembly Master Mix | Q5 Site-Directed Mutagenesis Kit (with competent cells) and Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) | EnGe Cas9 Nuclease | EnGen Mutation Detection Kit |
Integrated DNA technologies (IDT) | Human HPRT PCR Primer Mix, Mouse HPRT PCR Primer Mix, Nuclease Free Duplex Buffer | S.p. Cas9 Expression Plasmid | S.p. Cas9 Nuclease 3NLS (100, 500 μg) | CRISPR Negative Control crRNA, CRISPR Positive Control crRNA |
DNA 2.0 | Nickase Ninja All-in-One construct expressing specific dual gRNAs | Electra Cloning Kit | ||
Cyagen | ROSA26 large fragment knockin | |||
ORiGene | CRISPR/Cas starter kit (HA tagging human HSP60 at C-terminus). | pCas-Guide-Nickase (D10A), pT7-Cas9-Nickase (D10A) | pCas-Guide Cloning Kit, | pCas-Guide-scramble (also available as negative control) |
System Biosciences | Multiplex gRNA Cloning Kit Create CRISPR/Cas9 constructs with multiple gRNAs simultaneously for better genome editing | |||
Eurofins Genomics | Cloning Oligos | Indel Detection by Amplicon Analysis | Custom Sequencing (Check the sequence of your CRISPR plasmid or genomic target sequence) |
This table is provided only to facilitate researchers and R&D. It does not involve any commercial affiliation, marketing or financial purpose.