Figure 2.

Surgical specimens from case 15 in dose level-4 (DL-4) treated by three track injections with 3x10¹² vp of Ad-REIC. (a) Gross appearance of radical prostatectomy (RP) specimen sliced by a standard method. Cancer distribution areas are enclosed by the red line. Three yellow arrows indicate the Ad-REIC-targeted areas. (b) Gross appearance of hematoxylin and eosin (H&E)-stained histopathological sections. Cancer distribution areas are enclosed by the black line. Three blue circles indicate the targeted areas. (c) A photomicrograph of the targeted area from section no. 10, demonstrating tumor degeneration without significant tumor-infiltrating lymphocytes (TILs) (H&E). (d) Photomicrographs of the targeted area from the inner part of section no. 12. The inset shows low magnification view of pathological effects with remarkable TILs (H&E). Immunohistochemical staining, illustrating tumor-infiltrating CD8⁺ lymphocytes.