Figure 6.
Effects of Ef1 and S-Ef1 on TEER in IPEC-J2 cells. (a) Progression in TEER values of cells grown on Transwell filter for 10 days. (b) Polarised cells were untreated or treated with 5 × 107 CFU of Ef1 or S-Ef1 for 2 h and then stimulated with 5 × 107 CFU of K88ac for 0, 3, 6, and 12 h. The changes of TEER during incubation with bacteria were calculated based on the TEER values of PEC-J2 cells at 0 h. Data given are means (±SEM) of at least four separate experiments. The presence of various asterisks (∗ and ∗∗) indicates statistical differences with significant levels of p < 0.05 and p < 0.01, respectively.