Table 1.
Genes targeted by Cyp1b1 endogenous substrates and AhR. Separation by DMBA responses.
| Gene | WT (FC) | Cyp1b1-ko (FC) | Set | ||
|---|---|---|---|---|---|
| TCDD | DMBA | Control | DMBA | ||
| Stimulated | |||||
|
| |||||
| Cyp1b1 | 3.5 | 2.5 | n/a | n/a | A |
| Cyp1a1 | 9.7 | 15.0 | nd | [11.0]g | |
| Ahrr | 3.8 | 4.1 | nd | [10.0]g | |
|
| |||||
| IL6 | 3.0 | ns | 2.5 | 4.5 | B |
| Tnfa | 2.0 | ns | 1.5 | 3.0 | |
| Cxcl10a4 | 2.0 | ns | 1.5 | 4.0 | |
| Egr2a1 | 2.9 | ns | 2.5 | 4.4 | |
|
| |||||
| Hbb-b1a2 | 2.4 | 1.3 | 2.2 | ns | C |
| Fau | 2.0 | 1.5 | 2.2 | 1.3 | |
| Alas2b1 | 1.7 | ns | 1.6 | ns | |
| Creb3l3 | 2.9 | ns | 7.6 | 1.9 | |
| Hoxb6a5 | 2.6 | 1.3 | 2.7 | ns | |
| Sox19a6 | 1.5 | ns | 5.0 | 2.2 | |
| Polr2a | 5.0 | ns | 6.2 | 2.3 | |
|
| |||||
| Klf4 | 3.6 | 1.3 | 2.1 | 1.7 | D |
| Nr4a1 | 3.6 | ns | 2.0 | 2.2 | |
| Hspa-laa3 | 8.5 | ns | 2.6 | 3.0 | |
| Clqca7 | 1.8 | 1.4 | 2.8 | 2.4 | |
| Socs3 | 2.0 | 1.5 | 2.0 | 2.8 | |
|
| |||||
| Rag1 | −1.8 | ns | −3.0∗ | −3.0 | E |
| Erdr1 | 2.5 | ns | −2.4 | −2.4 | |
| Col5a3 | −1.5 | ns | −3.0∗ | −2.0 | |
| Faim3 | −1.5 | 1.3 | −2.9 | −1.5 | |
| Spp1 | −3.0 | 1.6 | −2.2 | −1.9 | |
Fold-changes (FC) are all significant; p < 0.05.
ns: not significant.
n/a: not available.
nd: not detectable.
[FC]g: relative to Cyp1b1-ko control genotype effect on PAH.
aComparable responses from family members (a1, Egr1; a2, Hbb-a1/a2; a3, Hspa-lb; a4, Cxcl9; a5, Hoxb9; a6, Sox15; a7, Gimap1).
bNo response from family member, Alas1.
∗No significant difference from BP.