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Basal mRNA expression of CYP1A2 (A), 2B6 (B), 2C9 (C), 2C19 (D), 2D6 (E), and 3A4 (F) in HepG2, HepaRG, 2D-, and 3D-cultured primary cryopreserved human hepatocytes. mRNA expression was determined by rt-PCR as described in the Materials and Methods Section. Results are expressed as mean ± SEM relative to GAPDH of at least three independent experiments. PHH, primary human hepatocytes.
