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. 2016 Nov 21;7:1700. doi: 10.3389/fpls.2016.01700

Table 3.

Comparison of N budget variables between the five categories (see column 1): grain and forage, and legume and non-legume crop combinations for those plots for which the N budget variables were high (see Table 2), “na,” denotes “non-applicable” comparisons.

N fertilizer (kg N ha−1) N fixed (kg N ha−1) N input (kg N ha−1) N output (kg N ha−1) N surplus (kg N ha−1) (N-out > 0) Biomass output (kg N ha−1) NUE (N-out > 0) BOE
Grain legume 0.36 ± 0.00 (1.43) 4.20 ± 0.06 (66.7) 4.68 ± 0.09 (107.8) 4.32 ± 0.11 (75.2) 6.98 ± 17.74 7.79 ± 0.20 (2416.3) 0.12 ± 0.36 (1.13) 3.57 ± 0.43 (35.5)
Grain non-legume 4.30 ± 0.03 (73.7) na 4.44 ± 0.08 (84.8) 4.06 ± 0.10 (58.0) −10.08 ± 16.93 8.36 ± 0.19 (4272.7) 0.75 ± 0.35 (2.12) 5.03 ± 0.42 (152.9)
Test na na 24.71 <0.0001 14.06 < 0.0001 7.96 < 0.0048 56.26 < 0.0001 38.23 < 0.0001 168.3664 < 0.0001
Grain mix 4.01 ± 0.04 (55.1) 4.19 ± 0.08 (66.0) 4.73 ± 0.13 (113.3) 4.70 ± 0.13 (109.9) −24.76 ± 19.84 8.10 ± 0.22 (3294.5) 0.15 ± 0.37 (1.16) 3.45 ± 0.43 (31.5)
Grain non-legume 4.30 ± 0.03 (73.7) na 4.33 ± 0.13 (75.9) 4.06 ± 0.09 (58.0) −12.74 ± 18.27 8.37 ± 0.19 (4315.6) 0.74 ± 0.34 (2.10) 5.04 ± 0.41 (154.5)
Test na na 61.0749 < 0.0001 41.15 < 0.0001 1.78 < 0.1820 4.45 < 0.0349 16.15 < 0.0001 78.07 < 0.0001
Forage mix 4.39 ± 0.18 (80.6) 4.75 ± 0.26 (115.6) 5.16 ± 0.14 (174.2) 4.75 ± 0.26 (115.6) −20.48 ± 20.80 8.61 ± 0.21 (5486.2) 0.66 ± 0.36 (1.93) 4.29 ± 0.28 (73.0)
Forage non-legume 4.95 ± 0.17 (141.2) 2.27 ± 0.33 (9.7) 4.93 ± 0.15 (138.4) 4.34 ± 0.26 (76.7) 28.04 ± 21.16 8.44 ± 0.21 (4628.6) 0.20 ± 0.36 (1.22) 4.16 ± 0.28 (64.1)
Test 19.77 < 0.0001 108.92 < 0.0001 8.30 < 0.0040 14.29 < 0.0001 33.81 < 0.0001 8.31 < 0.0039 24.867 < 0.0001 1.76 < 0.1851
Grain legume + forage 0.36 ± 0.00 (1.40) 4.23 ± 0.10 (68.7) 4.47 ± 0.04 (87.4) 4.66 ± 0.15 (105.6) −34.28 ± 4.27 8.00 ± 0.12 (2981.0) 0.34 ± 0.09 (1.40) 3.65 ± 0.09 (38.5)
Grain + forage na 3.14 ± 0.13 (23.0) 4.17 ± 0.04 (64.74) 4.04 ± 0.15 (56.8) −28.64 ± 3.31 8.22 ± 0.12 (3714.5) 0.83 ± 0.07 (2.29) 4.96 ± 0.07 (142.6)
Test na 37.24 < 0.0001 29.22 < 0.0001 120.21 < 0.0001 1.10 < 0.2940 22.67 < 0.0001 18.07 < 0.0001 98.91 < 0.0001
Grain + forage legume 4.13 ± 0.04 (67.2) 3.48 ± 0.58 (32.5) 4.29 ± 0.08 (73.0) 4.01 ± 0.17 (55.1) −8.66 ± 19.99 8.12 ± 0.11 (3361.0) 0.19 ± 0.19 (1.21) 4.32 ± 0.18 (75.2)
Grain + forage 3.95 ± 0.05 (49.4) 2.48 ± 0.60 (11.9) 4.03 ± 0.09 (56.3) 4.25 ± 0.18 (70.1) −57.78 ± 20.65 8.35 ± 0.12 (4230.2) 0.92 ± 0.22 (2.51) 5.05 ± 0.21 (156.0)
Test 14.69 < 0.0001 8.68 < 0.0032 17.52 < 0.0001 17.11 < 0.0001 45.03 < 0.0001 14.81 < 0.0001 26.84 < 0.0001 26.12 < 0.0001

F statistics and probability values obtained from analysis of variance are given in italics, non-italicized text are means ± standard errors. All data are log-transformed with the exception of N surplus, back transformed mean values are included in brackets. “NUE,” denotes nutrient use efficiency, and “BOE,” biomass output efficiency.