Table 2. Stability ranking of the candidate reference genes using five algorithms in different developmental stages of B. belcheri.
| Gene | RefFinder |
geNorm |
NormFinder |
BestKeeper |
deltaCt |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rank | GM | Rank | SV | Rank | SV | Rank | SV | Rank | SV | |
| S20 | 1 | 1.72 | 3 | 0.85 | 2 | 0.72 | 1 | 0.51 | 2 | 1.84 |
| 18 S | 2 | 2.23 | 1 | 0.76 | 3 | 0.76 | 2 | 0.55 | 5 | 1.89 |
| EF1A | 3 | 2.74 | 7 | 1.49 | 1 | 0.64 | 7 | 1.71 | 1 | 1.82 |
| CYC | 4 | 3.11 | 1 | 0.76 | 5 | 0.84 | 3 | 0.63 | 4 | 1.88 |
| G6PDH | 5 | 4.79 | 4 | 0.95 | 9 | 1.03 | 4 | 0.67 | 3 | 1.86 |
| UBC | 6 | 5.92 | 6 | 1.29 | 6 | 0.92 | 6 | 1.28 | 8 | 2.04 |
| L3 | 7 | 6.22 | 8 | 1.63 | 4 | 0.79 | 9 | 1.82 | 6 | 1.94 |
| TBP | 8 | 7.04 | 5 | 1.15 | 10 | 1.07 | 5 | 0.84 | 10 | 2.12 |
| TUB | 9 | 8.67 | 9 | 1.71 | 7 | 0.95 | 10 | 1.86 | 7 | 2.03 |
| GAPDH | 10 | 9.41 | 10 | 1.76 | 8 | 1.02 | 8 | 1.74 | 9 | 2.10 |
| HPRT | 11 | 11.37 | 11 | 1.85 | 12 | 1.46 | 11 | 1.89 | 12 | 2.59 |
| ACT | 12 | 12.26 | 12 | 1.99 | 11 | 1.41 | 12 | 2.59 | 11 | 2.54 |
| L13 | 13 | 12.93 | 13 | 2.03 | 13 | 1.71 | 13 | 2.87 | 13 | 2.84 |
Parameters of ranking were showed by using GM (geometric mean); SV, stability value. If no additional declarations, these abbreviations indicate the same means as above described in other tables.