Table 1. TCRβ retrogenic mice.
| TCR Name | TRBJ | CDR3 sequence | CNS shared (Total, n = 12) | CNS shared (Foxp3−) | CNS shared (Foxp3+) | SPL Shared (Total, n = 9) | SPL shared (Foxp3−) | SPL shared (Foxp3+) |
|---|---|---|---|---|---|---|---|---|
| Group 1, CNS-shared, public | ||||||||
| β1 | 2–1 | ASGETGGNYAEQF | 12 | 12 | 12 | 9 | 9 | 9 |
| β2 | 2–7 | ASGDRYEQY | 12 | 12 | 8 | 9 | 9 | 9 |
| β3 | 2–7 | ASGYEQY | 11 | 8 | 9 | 9 | 9 | 9 |
| β4 | 1–2 | ASGETANSDYT | 11 | 6 | 10 | 9 | 9 | 9 |
| β5 | 2–7 | ASGDAGGSYEQY | 10 | 8 | 10 | 9 | 9 | 9 |
| β6 | 2–7 | ASGDGEQY | 9 | 4 | 9 | 9 | 9 | 9 |
| Group 2, CNS non-shared, public | ||||||||
| β7 | 2–1 | ASGEQQGTEQF | 1 | 1 (22.6%) | 1 (2.5%) | 3 | 3 | 1 |
| β8 | 2–7 | ASGDGLGGSYEQY | 1 | 1 (11.7%) | 0 | 9 | 9 | 5 |
| β9 | 1–6 | ASGDVRGYNSPLY | 1 | 0 | 1 (4.4%) | 2 | 1 | 1 |
| β10 | 1–2 | ASGDGTSNSDYT | 1 | 0 | 1 (3.8%) | 9 | 9 | 2 |
| Group 3, non-shared, private | ||||||||
| β11 | 2–5 | ASGIGDTQY | 1 | 0 | 1 (7.3%) | 1 | 1 | 1 |
| β12 | 2–7 | ASGDAGTGYEQYF | ||||||
| β13 | 2–4 | ASGDWGGEDTLYF | ||||||
| β14 | 2–4 | ASGDETGGAYEQYF | ||||||
| β15 | 2–3 | ASGGGLGGTSAETLYF | ||||||
| Antigen non-specific negative control | ||||||||
| OTIIβ | 2–4 | ASSLGGESQNTLYF | ||||||
TRBV13-2+ TCRβ chains that were shared in the indicated number of total CD4+, CD4+Foxp3−, and CD4+Foxp3+ populations in the CNS’ and spleens of mice with EAE were transduced into TCRβ−/− HPCs to generate retrogenic mice. Sequences β1-β6 were identified in the CNS’ and spleens of multiple mice (CNS shared, public). Sequences β7-β10 were identified in the spleens of multiple mice, but in CNS tissue of only a single mouse (CNS non-shared, public). Sequence β11 was identified in the spleen and CNS of a single mouse, and sequences β12-β15 were isolated from TRBV13-2+ MOG35-55 -specific T cell hybridomas, and were not observed in any of the mice evaluated for the repertoire analyses (private). For TCRβ chains identified in a single mouse, the percent of total TRBV13-2+ TCR sequences in the CNS bearing the indicated sequence is listed in parentheses. OTIIβ comprises the TRBV13-2+ TCRβ chain from the OTII ovalbumin 323–229-specific TCR, and was assessed as a negative control.