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. 2016 Nov 21;6:37601. doi: 10.1038/srep37601

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Copyright © 2016, The Author(s)

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(A) Schematic of SNO-RAC, utilizing either immobilized double or triple mutants as indicated. (B) IoTrxs-SNO trans-nitrosylate GSNOR. Double mutant, C76S and C79S for IoTrx1; C35S and C38S for IoTrx3; triple mutant, C76S, C79S and C40S for IoTrx1; C35S, C38S and C60S for IoTrx3. (C) Yeast two-hybrid showing the interaction between GSNOR and IoTrx1 or IoTrx3 at dilution ratio of 1:500. (D) Coimmunoprecipitation by GSNOR monoclonal antibody or Trx antibody. The Upper lane shows immunoblotting of the proteins pulled down by GSNOR monoclonal antibody; lower lane immunoblotting of the proteins pulled down by commercialized monoclonal Trx antibody. Total, the amount of SNO (GSNOR-SNO) before denitrosylation; Residual, the amount of GSNOR-SNO after denitrosylation.